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ec growth medium mv hdmec  (PromoCell)


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    PromoCell ec growth medium mv hdmec
    EC nestin is regulated by laminar shear stress in vitro . Immunofluorescence staining of nestin in HUVEC, <t>HDMEC,</t> HCAEC and <t>HPAEC</t> <t>cultured</t> under ( A ) static conditions, or ( B ) 10 dyne/cm 2 laminar shear stress for 24 hours. Quantification of nestin spatial distribution in ( C ) HUVEC, ( D ) HDMEC, ( E ) HCAEC and ( F ) HPAEC. Each point represents an individual cell (n = 3–19 different experiments) Unpaired t-test *p-value < 0.05, **<0.01 ****<0.0001. ( G ) HUVEC were cultured under 10 dyne/cm 2 laminar shear stress for 0 h, 1 h, 4 h or 24 h, and stained for nestin, vimentin, actin, and DAPI. Pearson’s co-localisation value for nestin and vimentin is displayed on the top right of the merged images. ( H ) Quantification of nestin spatial distribution over the time course (n = 6); significance was calculated using one-way ANOVA. ( I ) NES and ( J ) VIM mRNA expression in EC under both static conditions and laminar shear stress, displayed relative to 18 s rRNA (n = 6–10). All graphs show means ± SD.
    Ec Growth Medium Mv Hdmec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 455 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ec growth medium mv hdmec/product/PromoCell
    Average 96 stars, based on 455 article reviews
    ec growth medium mv hdmec - by Bioz Stars, 2026-05
    96/100 stars

    Images

    1) Product Images from "A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein"

    Article Title: A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-32859-4

    EC nestin is regulated by laminar shear stress in vitro . Immunofluorescence staining of nestin in HUVEC, HDMEC, HCAEC and HPAEC cultured under ( A ) static conditions, or ( B ) 10 dyne/cm 2 laminar shear stress for 24 hours. Quantification of nestin spatial distribution in ( C ) HUVEC, ( D ) HDMEC, ( E ) HCAEC and ( F ) HPAEC. Each point represents an individual cell (n = 3–19 different experiments) Unpaired t-test *p-value < 0.05, **<0.01 ****<0.0001. ( G ) HUVEC were cultured under 10 dyne/cm 2 laminar shear stress for 0 h, 1 h, 4 h or 24 h, and stained for nestin, vimentin, actin, and DAPI. Pearson’s co-localisation value for nestin and vimentin is displayed on the top right of the merged images. ( H ) Quantification of nestin spatial distribution over the time course (n = 6); significance was calculated using one-way ANOVA. ( I ) NES and ( J ) VIM mRNA expression in EC under both static conditions and laminar shear stress, displayed relative to 18 s rRNA (n = 6–10). All graphs show means ± SD.
    Figure Legend Snippet: EC nestin is regulated by laminar shear stress in vitro . Immunofluorescence staining of nestin in HUVEC, HDMEC, HCAEC and HPAEC cultured under ( A ) static conditions, or ( B ) 10 dyne/cm 2 laminar shear stress for 24 hours. Quantification of nestin spatial distribution in ( C ) HUVEC, ( D ) HDMEC, ( E ) HCAEC and ( F ) HPAEC. Each point represents an individual cell (n = 3–19 different experiments) Unpaired t-test *p-value < 0.05, **<0.01 ****<0.0001. ( G ) HUVEC were cultured under 10 dyne/cm 2 laminar shear stress for 0 h, 1 h, 4 h or 24 h, and stained for nestin, vimentin, actin, and DAPI. Pearson’s co-localisation value for nestin and vimentin is displayed on the top right of the merged images. ( H ) Quantification of nestin spatial distribution over the time course (n = 6); significance was calculated using one-way ANOVA. ( I ) NES and ( J ) VIM mRNA expression in EC under both static conditions and laminar shear stress, displayed relative to 18 s rRNA (n = 6–10). All graphs show means ± SD.

    Techniques Used: In Vitro, Immunofluorescence, Staining, Cell Culture, Expressing



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    PromoCell ec growth medium mv hdmec
    EC nestin is regulated by laminar shear stress in vitro . Immunofluorescence staining of nestin in HUVEC, <t>HDMEC,</t> HCAEC and <t>HPAEC</t> <t>cultured</t> under ( A ) static conditions, or ( B ) 10 dyne/cm 2 laminar shear stress for 24 hours. Quantification of nestin spatial distribution in ( C ) HUVEC, ( D ) HDMEC, ( E ) HCAEC and ( F ) HPAEC. Each point represents an individual cell (n = 3–19 different experiments) Unpaired t-test *p-value < 0.05, **<0.01 ****<0.0001. ( G ) HUVEC were cultured under 10 dyne/cm 2 laminar shear stress for 0 h, 1 h, 4 h or 24 h, and stained for nestin, vimentin, actin, and DAPI. Pearson’s co-localisation value for nestin and vimentin is displayed on the top right of the merged images. ( H ) Quantification of nestin spatial distribution over the time course (n = 6); significance was calculated using one-way ANOVA. ( I ) NES and ( J ) VIM mRNA expression in EC under both static conditions and laminar shear stress, displayed relative to 18 s rRNA (n = 6–10). All graphs show means ± SD.
    Ec Growth Medium Mv Hdmec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ec growth medium mv hdmec/product/PromoCell
    Average 96 stars, based on 1 article reviews
    ec growth medium mv hdmec - by Bioz Stars, 2026-05
    96/100 stars
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    EC nestin is regulated by laminar shear stress in vitro . Immunofluorescence staining of nestin in HUVEC, HDMEC, HCAEC and HPAEC cultured under ( A ) static conditions, or ( B ) 10 dyne/cm 2 laminar shear stress for 24 hours. Quantification of nestin spatial distribution in ( C ) HUVEC, ( D ) HDMEC, ( E ) HCAEC and ( F ) HPAEC. Each point represents an individual cell (n = 3–19 different experiments) Unpaired t-test *p-value < 0.05, **<0.01 ****<0.0001. ( G ) HUVEC were cultured under 10 dyne/cm 2 laminar shear stress for 0 h, 1 h, 4 h or 24 h, and stained for nestin, vimentin, actin, and DAPI. Pearson’s co-localisation value for nestin and vimentin is displayed on the top right of the merged images. ( H ) Quantification of nestin spatial distribution over the time course (n = 6); significance was calculated using one-way ANOVA. ( I ) NES and ( J ) VIM mRNA expression in EC under both static conditions and laminar shear stress, displayed relative to 18 s rRNA (n = 6–10). All graphs show means ± SD.

    Journal: Scientific Reports

    Article Title: A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein

    doi: 10.1038/s41598-018-32859-4

    Figure Lengend Snippet: EC nestin is regulated by laminar shear stress in vitro . Immunofluorescence staining of nestin in HUVEC, HDMEC, HCAEC and HPAEC cultured under ( A ) static conditions, or ( B ) 10 dyne/cm 2 laminar shear stress for 24 hours. Quantification of nestin spatial distribution in ( C ) HUVEC, ( D ) HDMEC, ( E ) HCAEC and ( F ) HPAEC. Each point represents an individual cell (n = 3–19 different experiments) Unpaired t-test *p-value < 0.05, **<0.01 ****<0.0001. ( G ) HUVEC were cultured under 10 dyne/cm 2 laminar shear stress for 0 h, 1 h, 4 h or 24 h, and stained for nestin, vimentin, actin, and DAPI. Pearson’s co-localisation value for nestin and vimentin is displayed on the top right of the merged images. ( H ) Quantification of nestin spatial distribution over the time course (n = 6); significance was calculated using one-way ANOVA. ( I ) NES and ( J ) VIM mRNA expression in EC under both static conditions and laminar shear stress, displayed relative to 18 s rRNA (n = 6–10). All graphs show means ± SD.

    Article Snippet: Human Pulmonary Artery Endothelial Cells (HPAEC), Human Coronary Artery Endothelial Cells (HCAEC), and Human Dermal Microvascular Endothelial Cells (HDMEC) were obtained from Promocell in cryogenically frozen vials, and were cultured in EC Growth Medium (HPAEC, HCAEC) or EC Growth Medium-MV (HDMEC) (Promocell).

    Techniques: In Vitro, Immunofluorescence, Staining, Cell Culture, Expressing